Issue 95, 2018
From the journal:

Chemical Communications

A method to directly assay circRNA in real samples†

Jin Jiao,a   Tao Gao,a   Hai Shi,a   Anzhi Sheng,b   Yang Xiang,*a   Yongqian Shu*c  and  Genxi Li ORCID logo *ab  

* Corresponding authors

a State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, P. R. China
E-mail: xiangy@nju.edu.cn, genxili@nju.edu.cn

b Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, P. R. China

c Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P. R. China
E-mail: yongqianshu531@163.com

Abstract

A method to directly assay circular RNA (circRNA) is proposed in this work by utilizing the ‘microRNA (miRNA) sponge’ nature of circRNA and by taking the advantage of duplex-specific nuclease. Moreover, miRNA absorption site-mediated hairpin DNA unfolding and nuclease-assisted target recycling have also been designed in this method to amplify the signal readout, thus sensitive assay of circRNA can be achieved in real samples.

Graphical abstract: A method to directly assay circRNA in real samples

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Article information

DOI
https://doi.org/10.1039/C8CC08319C
Article type
Communication
Submitted
17 Oct 2018
Accepted
02 Nov 2018
First published
03 Nov 2018

Chem. Commun., 2018,54, 13451-13454

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A method to directly assay circRNA in real samples

J. Jiao, T. Gao, H. Shi, A. Sheng, Y. Xiang, Y. Shu and G. Li, Chem. Commun., 2018, 54, 13451 DOI: 10.1039/C8CC08319C

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