Crystallographic evidence for unexpected selective tyrosine hydroxylations in an aerated achiral Ru–papain conjugate†
Abstract
The X-ray structure of an aerated achiral Ru–papain conjugate has revealed the hydroxylation of two tyrosine residues found near the ruthenium ion. The most likely mechanism involves a ruthenium-bound superoxide as the reactive species responsible for the first hydroxylation and the resulting high valent Ru(IV)O species for the second one.