Efficient two-photon luminescence for cellular imaging using biocompatible nitrogen-doped graphene quantum dots conjugated with polymers

Ping-Ching Wu; Jiu-Yao Wang; Wen-Lung Wang; Chia-Yuan Chang; Chia-Hung Huang; Kun-Lin Yang; Jui-Cheng Chang; Chih-Li Lilian Hsu; Shih-Yao Chen; Ting-Mao Chou; Wen-Shuo Kuo

doi:10.1039/C7NR06836K

Efficient two-photon luminescence for cellular imaging using biocompatible nitrogen-doped graphene quantum dots conjugated with polymers†

Ping-Ching Wu,^a Jiu-Yao Wang,^bcd Wen-Lung Wang,^e Chia-Yuan Chang,^f Chia-Hung Huang,^gh Kun-Lin Yang,ⁱ Jui-Cheng Chang,^j Chih-Li Lilian Hsu,^d Shih-Yao Chen,*^e Ting-Mao Chou*^k and Wen-Shuo Kuo

*^bcl

Author affiliations

* Corresponding authors

^a Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

^b Allergy & Clinical Immunology Research Center, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
E-mail: wenshuokuo@mail.ncku.edu.tw

^c Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
E-mail: wenshuokuo@mail.ncku.edu.tw

^d Department of Microbiology & Immunology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

^e Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
E-mail: z9903038@email.ncku.edu.tw

^f Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

^g Metal Industries Research & Development Centre, Kaohsiung 811, Taiwan, Republic of China

^h Department of Materials Science Engineering, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

ⁱ Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung 802, Taiwan, Republic of China

^j Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

^k Division of Plastic Surgery, Department of Surgery, E-Da Hospital, Kaohsiung 824, Taiwan, Republic of China
E-mail: ed109842@edah.org.tw

^l Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
E-mail: wenshuokuo@mail.ncku.edu.tw

Abstract

Nitrogen-doped graphene quantum dot (N-GQD) nanomaterials conjugated with polyethylenimine (PEI)-polystyrene sulfonate (PSS)-anti-epidermal growth factor receptor (Ab_EGFR) antibody (N-GQD-PEI-PSS-Ab_EGFR) demonstrated impressive two-photon properties and stability, signifying that they can serve as an effective two-photon contrast agent in two-photon bioimaging. Furthermore, they provided high intensity, brightness, and signal-to-noise ratios at an ultra-low two-photon excitation (TPE) power level in an observation extending to a deep, three-dimensional depth.

Nanoscale

Efficient two-photon luminescence for cellular imaging using biocompatible nitrogen-doped graphene quantum dots conjugated with polymers†

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Efficient two-photon luminescence for cellular imaging using biocompatible nitrogen-doped graphene quantum dots conjugated with polymers

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