Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field

Saira F. Bakshi; Nataliia Guz; Andrey Zakharchenko; Han Deng; Alexei V. Tumanov; Craig D. Woodworth; Sergiy Minko; Dmitry M. Kolpashchikov; Evgeny Katz

doi:10.1039/C7NR08581H

Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field†

Saira F. Bakshi,

^a Nataliia Guz,

^a Andrey Zakharchenko,

^b Han Deng,

^c Alexei V. Tumanov,

^d Craig D. Woodworth,

^c Sergiy Minko,

*^b Dmitry M. Kolpashchikov

*^ef and Evgeny Katz

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA
E-mail: ekatz@clarkson.edu

^b Nanostructured Materials Lab, University of Georgia, Athens, GA 30602, USA
E-mail: sminko@uga.edu

^c Department of Biology, Clarkson University, Potsdam, NY 13699-5810, USA

^d Department of Microbiology, Immunology, and Molecular Genetics, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900, USA

^e Chemistry Department, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816-2366, USA
E-mail: dmitry.kolpashchikov@ucf.edu

^f ITMO University, Laboratory of Solution Chemistry of Advanced Materials and Technologies, Lomonosova St. 9, St Petersburg, Russian Federation

Abstract

A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular nanoreactor features two species of magnetic particles, each functionalized with two components of a binary deoxyribozyme system. In the presence of a target mRNA analyte and a magnetic field, the nanoreactor is assembled to form a biocompartment enclosed by the polymeric brush that enables catalytic function of the binary deoxyribozyme with enhanced kinetics. MaBiDz was demonstrated here as a cellular sensor for rapid detection and imaging of a target mRNA biomarker for metastatic breast cancer, and its function shows potential to be expanded as a biomimetic organelle that can downregulate the activity of a target mRNA biomarker.

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Article information

DOI: https://doi.org/10.1039/C7NR08581H
Article type: Paper
Submitted: 16 Nov 2017
Accepted: 18 Dec 2017
First published: 18 Dec 2017

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Nanoscale, 2018,10, 1356-1365

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Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field

S. F. Bakshi, N. Guz, A. Zakharchenko, H. Deng, A. V. Tumanov, C. D. Woodworth, S. Minko, D. M. Kolpashchikov and E. Katz, Nanoscale, 2018, 10, 1356 DOI: 10.1039/C7NR08581H

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Nanoscale

Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field†

Abstract

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Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field

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