Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering

Dolev Rimmerman; Denis Leshchev; Darren J. Hsu; Jiyun Hong; Baxter Abraham; Irina Kosheleva; Robert Henning; Lin X. Chen

doi:10.1039/C8PP00034D

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Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering†

Dolev Rimmerman,^a Denis Leshchev,^a Darren J. Hsu,^a Jiyun Hong,^a Baxter Abraham,^b Irina Kosheleva,^c Robert Henning^c and Lin X. Chen

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* Corresponding authors

^a Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA
E-mail: l-chen@northwestern.edu

^b Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA

^c Center for Advanced Radiation Sources, The University of Chicago, Illinois 60637, USA

^d Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, Illinois 60439, USA
E-mail: lchen@anl.gov

Abstract

The structural dynamics of insulin hexamer dissociation were studied by the photoinduced temperature jump technique and monitored by time-resolved X-ray scattering. The process of hexamer dissociation was found to involve several transient intermediates, including an expanded hexamer and an unstable tetramer. Our findings provide insights into the mechanisms of protien–protein association.

This article is part of the themed collections: Celebrating Excellence in Research: 100 Women of Chemistry and 28th International Conference on Photochemistry (ICP 2017)

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DOI: https://doi.org/10.1039/C8PP00034D
Article type: Communication
Submitted: 25 Jan 2018
Accepted: 24 May 2018
First published: 25 May 2018

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Photochem. Photobiol. Sci., 2018,17, 874-882

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Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering

D. Rimmerman, D. Leshchev, D. J. Hsu, J. Hong, B. Abraham, I. Kosheleva, R. Henning and L. X. Chen, Photochem. Photobiol. Sci., 2018, 17, 874 DOI: 10.1039/C8PP00034D

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Photochemical & Photobiological Sciences

Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering†

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Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering

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