A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

Haibo Xing; Wenchao Gu; Dang Xu; Fuxiang Tian; Linyun Yao; Zhenwei Wang; Xiaojun Hu

doi:10.1039/C7RA12970J

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A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

Haibo Xing,

†^a Wenchao Gu,†^c Dang Xu,^a Fuxiang Tian,^a Linyun Yao,^b Zhenwei Wang^a and Xiaojun Hu

*^a

Author affiliations

* Corresponding authors

^a School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China
E-mail: 267358918@qq.com

^b School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, China

^c Putuo District Center for Disease Control and Prevention, Shanghai, China

Abstract

A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be determined by fluorescence quenching. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 0.68 ppb and a detection range from 0 to 200 ppb.

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DOI: https://doi.org/10.1039/C7RA12970J
Article type: Paper
Submitted: 01 Dec 2017
Accepted: 26 Dec 2017
First published: 10 Jan 2018
This article is Open Access

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RSC Adv., 2018,8, 2418-2425

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A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

H. Xing, W. Gu, D. Xu, F. Tian, L. Yao, Z. Wang and X. Hu, RSC Adv., 2018, 8, 2418 DOI: 10.1039/C7RA12970J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

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A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

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