Issue 20, 2019
From the journal:

Chemical Communications

A novel template repairing-PCR (TR-PCR) reaction platform for microRNA detection using translesional synthesis on DNA templates containing abasic sites†

Dianming Zhou,‡a   Xiaohui Lin,‡a   Weichen Gao,b   Jiafang Piao,b   Shufei Li,a   Ning He,a   Zhiyong Qian,*a   Miao Zhao*a  and  Xiaoqun Gong ORCID logo *b  

* Corresponding authors

a Department of Toxicology, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China
E-mail: 13323360260@163.com, zmxb129@163.com

b School of Life Sciences, Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Tianjin 300072, China
E-mail: gongxiaoqun@tju.edu.cn

Abstract

A novel template repairing-PCR technology based on miRNA-primed bypass synthesis at the abasic sites on the PCR template is developed as a sensitive and selective platform for miRNA detection. The assay is expected to show great promise for reverse transcription-free RNA PCR amplification and target-based coding analysis.

Graphical abstract: A novel template repairing-PCR (TR-PCR) reaction platform for microRNA detection using translesional synthesis on DNA templates containing abasic sites

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Article information

DOI
https://doi.org/10.1039/C8CC10226K
Article type
Communication
Submitted
27 Dec 2018
Accepted
06 Feb 2019
First published
06 Feb 2019

Chem. Commun., 2019,55, 2932-2935

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A novel template repairing-PCR (TR-PCR) reaction platform for microRNA detection using translesional synthesis on DNA templates containing abasic sites

D. Zhou, X. Lin, W. Gao, J. Piao, S. Li, N. He, Z. Qian, M. Zhao and X. Gong, Chem. Commun., 2019, 55, 2932 DOI: 10.1039/C8CC10226K

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