Issue 38, 2019

Chiral separation of d/l-arginine with whole cells through an engineered FhuA nanochannel

Abstract

Downstream processing to obtain enantiopure compounds from a racemic mixture relies mainly on crystallization. Natural transporters can specifically translocate enantiomers through membranes. Here a β-barrel transmembrane protein FhuA is re-engineered into a chiral channel protein (FhuAF4) to resolve racemic mixtures of D-/L-arginine. The engineered FhuAF4 variant exhibits an enantioselectivity (E-value) of 1.92 and an enantiomeric excess percentage (ee%) of 23.91 at 52.39% conversion. OmniChange mutant libraries at the computationally identified “filter-regions” likely help to identify FhuA variants for enantiomeric separation of other compounds.

Graphical abstract: Chiral separation of d/l-arginine with whole cells through an engineered FhuA nanochannel

Supplementary files

Article information

Article type
Communication
Submitted
07 Jan 2019
Accepted
19 Mar 2019
First published
21 Mar 2019

Chem. Commun., 2019,55, 5431-5434

Chiral separation of D/L-arginine with whole cells through an engineered FhuA nanochannel

D. Anand, G. V. Dhoke, J. Gehrmann, T. M. Garakani, M. D. Davari, M. Bocola, L. Zhu and U. Schwaneberg, Chem. Commun., 2019, 55, 5431 DOI: 10.1039/C9CC00154A

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