Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

Adam M. Thomas; Sanja Brolih; Joanna F. McGouran; Afaf H. El-Sagheer; Denis Ptchelkine; Morgan Jones; Neil Q. McDonald; Peter J. McHugh; Tom Brown

doi:10.1039/C9CC05476F

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Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors†

Adam M. Thomas,

‡^abc Sanja Brolih,‡^b Joanna F. McGouran,

‡§^a Afaf H. El-Sagheer,

^ad Denis Ptchelkine,^b Morgan Jones,^c Neil Q. McDonald,*^ce Peter J. McHugh

*^b and Tom Brown

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, UK
E-mail: tom.brown@chem.ox.ac.uk
Tel: +44 01865 275413

^b Department of Oncology, MRC-Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK

^c The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK

^d Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt

^e Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, Malet Street, London WC1E 7HX, UK

Abstract

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

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Article information

DOI: https://doi.org/10.1039/C9CC05476F
Article type: Communication
Submitted: 16 Jul 2019
Accepted: 15 Aug 2019
First published: 09 Sep 2019
This article is Open Access

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Chem. Commun., 2019,55, 11671-11674

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Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

A. M. Thomas, S. Brolih, J. F. McGouran, A. H. El-Sagheer, D. Ptchelkine, M. Jones, N. Q. McDonald, P. J. McHugh and T. Brown, Chem. Commun., 2019, 55, 11671 DOI: 10.1039/C9CC05476F

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Chemical Communications

Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors†

Abstract

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Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

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