Issue 1, 2019

An enhanced enzymatic reaction using a triphase system based on superhydrophobic mesoporous nanowire arrays

Abstract

Gaseous reactants play a key role in a wide range of biocatalytic reactions, however reaction kinetics are generally limited by the slow mass transport of gases (typically oxygen) in or through aqueous solutions. Inspired by the morphologies of natural non-wetting surfaces, herein we address this limitation by developing a triphase reaction system possessing a triphase gas–solid–liquid interface. As a proof of concept, we study the kinetics of glucose oxidase (GOx) catalyzed reactions using a triphase system fabricated by layering GOx upon superhydrophobic mesoporous ZnO nanowire arrays through which oxygen, needed for the enzymatic reaction, is supplied directly from the atmosphere to the liquid–solid interface. We find that the enzymatic reaction rate is enhanced by a factor of 30 over that obtained from a conventional diphase system where oxygen is supplied through and from the liquid. The triphase system offers the opportunity to develop high performance bioassay systems, serving as an enabling platform for addressing challenges posed by gas-deficit kinetics.

Graphical abstract: An enhanced enzymatic reaction using a triphase system based on superhydrophobic mesoporous nanowire arrays

Supplementary files

Article information

Article type
Communication
Submitted
14 Jul 2018
Accepted
27 Sep 2018
First published
27 Sep 2018

Nanoscale Horiz., 2019,4, 231-235

An enhanced enzymatic reaction using a triphase system based on superhydrophobic mesoporous nanowire arrays

F. Guan, J. Zhang, H. Tang, L. Chen and X. Feng, Nanoscale Horiz., 2019, 4, 231 DOI: 10.1039/C8NH00184G

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