Translocation of tetrahedral DNA nanostructures through a solid-state nanopore

Xinjia Zhao; Ruiping Ma; Ying Hu; Xiaoyu Chen; Ruifen Dou; Ke Liu; Chengjun Cui; Huajie Liu; Qian Li; Dun Pan; Xinyan Shan; Lihua Wang; Chunhai Fan; Xinghua Lu

doi:10.1039/C8NR10474C

Translocation of tetrahedral DNA nanostructures through a solid-state nanopore†

Xinjia Zhao,

^ab Ruiping Ma,^c Ying Hu,^a Xiaoyu Chen,^ab Ruifen Dou,^c Ke Liu,^d Chengjun Cui,^e Huajie Liu,

^e Qian Li,

^d Dun Pan,^ef Xinyan Shan,*^a Lihua Wang,

*^eg Chunhai Fan

^ed and Xinghua Lu

*^abhi

Author affiliations

* Corresponding authors

^a Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
E-mail: xhlu@aphy.iphy.ac.cn, shanxinyan@aphy.iphy.ac.cn

^b School of Physical Sciences, University of Chinese Academy of Sciences, Beijing 100190, China

^c Beijing Normal University, 100000 Beijing, China

^d School of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China

^e Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
E-mail: wanglihua@sinap.ac.cn

^f Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200030, China

^g Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, China

^h Center for Excellence in Topological Quantum Computation, Chinese Academy of Sciences, Beijing 100190, China

ⁱ Songshan Lake Materials Laboratory, Dongguan, Guangdong 523808, China

Abstract

Tetrahedral DNA nanostructures (TDNs) are programmable DNA nanostructures that have great potential in bio-sensing, cell imaging and therapeutic applications. In this study, we investigate the translocation behavior of individual TDNs through solid-state nanopores. Pronounced translocation signals for TDNs are observed that are sensitive to the size of the nanostructures. TDNs bound to linear DNA molecules produce an extra signal in the ionic current traces. Statistical analysis of its relative temporal position reveals distinct features between TDNs bound to the end and those bound to the middle of the linear DNA molecules. A featured current trace for two TDNs bound to the same linear DNA molecule has also been observed. Our study demonstrates the potential of using TDNs as sensitive bio-sensors to detect specific segments of a single DNA molecule in real time, based on solid-state nanopore devices.

Nanoscale

Translocation of tetrahedral DNA nanostructures through a solid-state nanopore†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Translocation of tetrahedral DNA nanostructures through a solid-state nanopore

Social activity

Search articles by author

Spotlight

Advertisements