Potentiating photodynamic therapy of ICG-loaded nanoparticles by depleting GSH with PEITC

Hang Hu; Jitang Chen; Hai Yang; Xuetao Huang; Honglian Wu; Yuxin Wu; Fenfang Li; Yunfei Yi; Chen Xiao; Yihui Li; Yuxiang Tang; Zifu Li; Bixiang Zhang; Xiangliang Yang

doi:10.1039/C9NR01306G

Potentiating photodynamic therapy of ICG-loaded nanoparticles by depleting GSH with PEITC†

Hang Hu,‡^ab Jitang Chen,‡^ab Hai Yang,‡^ab Xuetao Huang,^ab Honglian Wu,^ab Yuxin Wu,^ab Fenfang Li,^ab Yunfei Yi,^ab Chen Xiao,^ab Yihui Li,^ab Yuxiang Tang,^ab Zifu Li,

*^abcd Bixiang Zhang*^e and Xiangliang Yang*^abc

Author affiliations

* Corresponding authors

^a National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China

^b Department of Nanomedicine and Biopharmaceutics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China

^c Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Huazhong University of Science and Technology, Wuhan, China
E-mail: yangxl@hust.edu.cn
Fax: +86 27 87792234

^d Wuhan Institute of Biotechnology, High Tech Road 666, East Lake High Tech Zone, Wuhan, China
E-mail: zifuli@hust.edu.cn
Fax: +86 27 87792234

^e Institute of Hepato-Pancreato-Bililary Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
E-mail: bixiangzhang@163.com
Fax: +86 27 83662851

Abstract

Photodynamic therapy (PDT) is a clinically approved cancer treatment which utilizes reactive oxygen species (ROS) to eradicate cancer cells. But the high concentration of GSH inside tumor cells can neutralize the generated ROS during PDT, resulting in an insufficient therapeutic effect. To address this issue, we combined ICG-loaded nanoparticles with PEITC for potent PDT. ICG encapsulated in novel hydroxyethyl starch-oleic acid conjugate (HES-OA) nanoparticles (∼50 nm) exhibited excellent stability and efficient singlet oxygen generation under laser irradiation, promoted cellular uptake, and enhanced tumor accumulation, whilst PEITC depleted intracellular GSH significantly. As a result, PDT based on ICG-loaded NPs combined with PEITC synergistically suppressed cancer cells both in vitro and in vivo. Potentiating ICG-loaded NPs with PEITC represents a novel and efficient strategy to enhance PDT efficacy.

Nanoscale

Potentiating photodynamic therapy of ICG-loaded nanoparticles by depleting GSH with PEITC†

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Potentiating photodynamic therapy of ICG-loaded nanoparticles by depleting GSH with PEITC

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