A positron-emission tomography (PET)/magnetic resonance imaging (MRI) platform to track in vivo small extracellular vesicles

Abstract

Here we report a two-step surface modification methodology to radiolabel small extracellular vesicles (SEVs) with ⁶⁴CuCl₂ for PET/MRI imaging. The modification did not change or damage the morphology, surface receptor proteins and internal RNA content. Radiolabeled SEVs could be detected in organs with low accumulation such as the brain (0.4–0.5% ID g⁻¹) and their brain location determined by MRI.