Enhanced bactericidal efficacy of polymer stabilized silver nanoparticles in conjugation with different classes of antibiotics

Abstract

The paper presents the interaction mechanism of silver nanoparticles (AgNPs) with different antibiotics and the antibacterial efficacy of the formed conjugates. The AgNPs used in this study were synthesized from silver nitrate using sodium borohydride as a reducing agent, in the presence of PVP as a protecting agent. Two antibiotics, amikacin and vancomycin with different modes of action, were used to functionalize the synthesized PVP-capped AgNPs. The formation of antibiotic-AgNPs conjugate was confirmed by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and X-ray diffraction (XRD) and the results suggest the conjugation of both drugs to silver nanoparticle surfaces. FTIR results indicate that intermolecular hydrogen bonding exists between PVP-coated AgNPs and antibiotics. The oxygen atom coordinated with PVP was available for interaction with either amine or amide groups of drugs. Further, the antibacterial efficacy of these PVP-capped AgNPs with selected antibiotics was evaluated against Escherichia coli and Staphylococcus aureus by agar well diffusion test. Synergetic bactericidal activity for antibiotic-AgNPs conjugate was observed against both microbes.