Issue 2, 2019, Issue in Progress

Self-organized nanocrystal rings formed by microemulsion for selective recognition of proteins and immunoassays

Abstract

A simple and cheap method to fabricate a nanocrystal ring pattern was developed by utilization of a microemulsion in this study. The mixture of polystyrene and stabilizer dichloromethane solution that contained nanocrystal aqueous solution, prepared through shaking, was applied to fabricate a reverse microemulsion. After spreading and evaporating the solvent of microemulsion on a glass slide, an ordered honeycomb film was produced, accompanied by the formation of a nanocrystal ring pattern. The nanocrystal pattern could be readily applied for immunoassays and recognition of proteins. The pattern with antibody marked by a green colored nanocrystal specifically bound with antigen labeled by a red colored nanocrystal, leading to the enhancement in red fluorescent ring pattern and decrease in green fluorescent pattern. When the unlabeled antigen was added, the green fluorescent pattern was recovered. In addition, the ring pattern with immunocomplex could selectively recognize antigen and transferrin proteins. This strategy reveals that these patterns have potential applications in biochips, biosensors, imaging analysis and so forth.

Graphical abstract: Self-organized nanocrystal rings formed by microemulsion for selective recognition of proteins and immunoassays

Supplementary files

Article information

Article type
Paper
Submitted
24 Nov 2018
Accepted
10 Dec 2018
First published
04 Jan 2019
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 699-703

Self-organized nanocrystal rings formed by microemulsion for selective recognition of proteins and immunoassays

J. Liang, L. Yu, Z. Lin, K. Song, J. Zhang and J. Zhang, RSC Adv., 2019, 9, 699 DOI: 10.1039/C8RA09662G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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