Effects of pH on the biodegradation characteristics of thermophilic micro-aerobic digestion for sludge stabilization

Abstract

For a thermophilic micro-aerobic digestion (TMAD) system, the pH is closely related to the production of short-chain fatty acids and ammonia release. Batch experiments at different pH values were carried out to elucidate the effects of pH on the biodegradation characteristics of TMAD processes. A digester at pH 9.0 maintained better performance of sludge stabilization than acidic and neutral digestion systems, and the reduction of volatile solids reached ≤41.3% on day-10. We proposed a pathway of proton transfer in the TMAD system. An acidic digestion environment, in general, resulted in K⁺ deficiency, low enzyme activity, and oxidative stress. An alkaline digestion environment facilitated substrate oxidation, accelerated humification, and prevented the inhibition caused by the accumulated acids or ammonia nitrogen. The pH and digestion time affected the microbial population and species richness significantly. An alkaline digestion system presented lower bacterial diversity and maintained a higher richness of functional microbes such as Paenalcaligenes and Pseudogracilibacillus. Hence, an alkaline digestion system maintained a relatively high oxidase activity, and mitigated the potential oxidative stress for thermophiles.