A novel, fast, high sensitivity biosensor for supporting therapeutic decisions and onset actions for chest pain cases

Abstract

In this work, a novel and promising organic nano linker (NL) was prepared via refluxing 5-aminoisophthalic acid and 1,2-phenylenediamine at 80 °C for 48 h. After that, this linker was reacted with manganese chloride to afford a novel manganese metal–organic framework (Mn–MOF). The produced materials were characterized using 1H-NMR, 13C-NMR, mass spectrometry, elemental analysis, UV, IR, FE-SEM, EDX, TEM, and thermal study. In addition to X-ray diffraction, XPS, magnetic properties and photoluminescence investigation for Mn–MOF. The study was extended to apply Mn–MOF as electroactive material for the preparation of a novel cardiac troponin I (cTn) potentiometric membrane biosensor. The biosensor, based on Mn–MOF with an optimized membrane composition, exhibits a fast, stable and linear-Nernstian response to cTn in the concentration range between 0.01 and 30.0 ng mL⁻¹ with a pH range between 5.6 and 10.1 and a fast response time of 20 ± 5 s. The detection and quantification limits are 0.055 and, 0.168 ng mL⁻¹, respectively. The lifetime of the electrode is between 3–12 week without a significant change in the membrane compositions and the performance characteristics based on the storage conditions. The electrode shows high selectivity towards cTn with respect to common interfering analytes. This approach of Mn–MOF-electrode could be addressed, facilitated and helped an emergency departments (EDs) decision-making in patients with chest pain and early myocardial infarction diagnosis. The future vision is converting the present approach to a small device with satisfactory results which will be used in term of point-of-care testing (POCT) for measuring the most important cardiac blood biomarkers.