Issue 43, 2019

A fluorescent nanoparticle probe based on sugar-substituted tetraphenylethene for label-free detection of galectin-3

Abstract

Galectin-3 is a vital biomarker for cancer progression. An aggregation-induced emission (AIE)-active nanoparticle probe is designed and synthesized for label-free detection of galectin-3 based on carbohydrate–protein interactions. The probe employs tetraphenylethene (TPE) as the fluorescent core structure and N-acetyllactosamine (LacNAc) as galactoside residues and self-aggregates into uniform nanoparticles with multiple binding sites on the surface targeting galectin-3. Upon crosslinking of the nanoparticles and galectin-3, large complexes are formed and a significant fluorescence change is measured, allowing rapid, sensitive and specific detection of galectin-3 via a fluorometric assay. Its demonstrated superior anti-interference ability and applicability in serum samples offer promising applications in cancer diagnoses.

Graphical abstract: A fluorescent nanoparticle probe based on sugar-substituted tetraphenylethene for label-free detection of galectin-3

Supplementary files

Article information

Article type
Communication
Submitted
11 Aug 2019
Accepted
15 Oct 2019
First published
16 Oct 2019

J. Mater. Chem. B, 2019,7, 6737-6741

A fluorescent nanoparticle probe based on sugar-substituted tetraphenylethene for label-free detection of galectin-3

Z. Zhang, Y. Yu, Y. Zhao and K. M. Ng, J. Mater. Chem. B, 2019, 7, 6737 DOI: 10.1039/C9TB01703H

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