Molecular isomerization triggered by H2S to an NIR accessible first direct visualization of Ca2+-dependent production in living HeLa cells

Abstract

Few studies determined the role of intracellular labile Ca²⁺ in H₂S homeostasis. Undoubtedly, fluorescent probes are powerful tools for exploring the question because of their unique advantages: non-destruction, visualization, and multi-levels imaging. Herein, a near-infrared (λ_em = 687 nm) and methylene blue chromophore-based fluorescent probe (MB1) for H₂S was rationally developed. Based on its high sensitivity and selectivity, MB1 was employed to image the concentration change of H₂S, upon stimulating it with ionomycin (a specific calcium ionophore). We found that the intracellular labile Ca²⁺ acted as a promotor for H₂S production in living cells. Furthermore, cystathionine γ-lyase (CSE) might have functioned as a positive mediator of Ca²⁺-dependent H₂S production. These direct and visible links for H₂S/Ca²⁺ will help us to understand the complex signaling in a better way.