Issue 22, 2020

Colorimetric detection of glucose based on the binding specificity of a synthetic cyclic peptide

Abstract

A novel colorimetric sensing method for glucose was developed based on the catalytic activity of Au nanoparticles (NPs) and a synthetic cyclic peptide that specifically binds with glucose. It is the first time that a cyclic peptide was used as a recognition element for glucose sensing. In the absence of glucose, the monolayers of cyclic peptide on the Au NP surfaces interfered little with the adsorption of 4-nitrophenol, and the Au NPs catalyze the reduction of bright yellow 4-nitrophenol to colorless 4-aminophenol in the presence of NaBH4. Added glucose was preferentially bound by the cyclic peptides and impeded the adsorption of 4-nitrophenol. Therefore, the color of the solution presented varying shades of yellow depending on the concentration of glucose. The method had a short response time of 10 min and demonstrated a linear response over a range of glucose concentrations from 0.1 mM to 20 mM, with a lower limit of detection of 0.04 mM. Meanwhile, it also provided results readily observable by the naked eye. The method was successfully applied for the detection of glucose in spiked food samples (Chinese cabbage, pear, and wheat flour) and spiked rabbit blood, and a good recovery rate of 88.04–103.28% and 94.27–101.53% was obtained, respectively.

Graphical abstract: Colorimetric detection of glucose based on the binding specificity of a synthetic cyclic peptide

Article information

Article type
Paper
Submitted
31 Jan 2020
Accepted
26 Jul 2020
First published
03 Aug 2020

Analyst, 2020,145, 7234-7241

Colorimetric detection of glucose based on the binding specificity of a synthetic cyclic peptide

X. Wu, J. Yin, J. Liu, Y. Gu, S. Wang and J. Wang, Analyst, 2020, 145, 7234 DOI: 10.1039/D0AN00211A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements