Issue 23, 2020

A gas pressure and colorimetric signal dual-mode strategy for sensitive detection of spermine using ssDNA-coated Au@Pt nanoparticles as the probe

Abstract

The naturally occurring spermine (Spm), as one of the many cationic polyamines, plays a key role in biological processes and is involved in a variety of diseases. The very similar structures among biogenic polyamines present a major challenge to achieve discriminative testing among them. In this contribution, using arbitrary ssDNA-coated Au@PtNPs as the probe, we demonstrated that a dual-mode strategy via a gas pressure and colorimetric signal readout can be used for the sensitive and specific detection of Spm, due to the target-responsive aggregation of the Au@PtNPs leading to the inhibition of the catalyzed gas-generation reaction and the colorimetric change of the Au@PtNP solution. The proposed pressure-based signaling strategy has a detection limit of 9.6 nM, and can be used not only in the laboratory but also in the point-of-care setting. Meanwhile, the colorimetric assay displays the advantage of being easily discerned with the naked eye. Compared with the traditional methods of chromatography and capillary electrophoresis combined with chemical derivatization, the strategy described here would provide a convenient new alternative for the specific detection of Spm in biological samples.

Graphical abstract: A gas pressure and colorimetric signal dual-mode strategy for sensitive detection of spermine using ssDNA-coated Au@Pt nanoparticles as the probe

Supplementary files

Article information

Article type
Paper
Submitted
26 Jul 2020
Accepted
27 Aug 2020
First published
24 Sep 2020

Analyst, 2020,145, 7673-7679

A gas pressure and colorimetric signal dual-mode strategy for sensitive detection of spermine using ssDNA-coated Au@Pt nanoparticles as the probe

H. Jiang, X. Rao, L. Li and Z. Liu, Analyst, 2020, 145, 7673 DOI: 10.1039/D0AN01473G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements