Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

Jintao Zhao; Yuan Qu; Hao Gao; Miao Zhong; Xinming Li; Fang Zhang; Yaxiong Chen; Lu Gan; Guodong Hu; Hong Zhang; Shengxiang Zhang; Jianguo Fang

doi:10.1039/D0CC05900E

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe†

Jintao Zhao,

‡^a Yuan Qu,‡^b Hao Gao,^b Miao Zhong,^a Xinming Li,^ac Fang Zhang,^a Yaxiong Chen,^ad Lu Gan,^d Guodong Hu,^a Hong Zhang,

^d Shengxiang Zhang*^b and Jianguo Fang

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China
E-mail: fangjg@lzu.edu.cn

^b Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
E-mail: sxzhang@lzu.edu.cn

^c State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

^d Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, China

Abstract

Thioredoxin reductase (TrxR) enzymes are critical in regulating redox homeostasis in cells. We report the first two-photon fluorescent probe of mammalian TrxR (TP-TRFS). TP-TRFS retains high specificity in recognizing TrxR. More importantly, the two-photon absorbing character of TP-TRFS enables it to be used in vivo. With the aid of TP-TRFS, a remarkable decline of the TrxR function was observed in the brain of a mouse model of stroke for the first time, providing a mechanistic link of TrxR dysfunction with stroke.

Chemical Communications

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

Social activity

Search articles by author

Spotlight

Advertisements