Detection of an alcohol-associated cancer marker by single-molecule quantum sequencing

Abstract

Alcoholic beverages are a well-known risk factor for cancer. N²-Ethyl-2′-deoxyguanosine (N²-Et-dG) is a promising biomarker for alcohol-associated cancers. However, the lack of a convenient detection method for N²-Et-dG hinders the development of practical DNA damage markers. Herein, we develop a detection method for N²-Et-dG using a single-molecule quantum sequencing (SMQS) method and machine learning analysis. Our method succeeded in discriminating between N²-Et-dG and dG with an accuracy of 99%, using 20 signals. Our developed method quantified the mixing ratio of N²-Et-dG from a mixed solution of N²-Et-dG and dG. It is shown that our method has the potential to facilitate the development of DNA damage markers, and thus the early detection and prevention of cancers.