Issue 5, 2020

Oleuropein and its peracetylated derivative negatively regulate osteoclastogenesis by controlling the expression of genes involved in osteoclast differentiation and function

Abstract

During chronic inflammation, macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) have well established effects on gene networks that stimulate osteoclastogenesis, which is the culprit of several bone diseases. In this study, we investigated the anti-osteoclastogenic effects in vitro of oleuropein (OL) and its peracetylated derivative (Per-OL) by exploring the expression level of key hub genes involved in fate decision and lineage commitment, differentiation, and function of human blood monocyte-derived osteoclasts. Monocytes were purified from peripheral blood mononuclear cells of healthy individuals using commercial antibodies coated with magnetic beads and treated with M-CSF/RANKL in the presence or absence of OL or Per-OL (25 and 50 μM) for 6 days. We demonstrated that OL and especially Per-OL impair transcriptional gene circuits able to support osteoclastogenesis from human blood monocytes. Our results indicate that OL and notably Per-OL are promising candidates to control osteoclastogenesis.

Graphical abstract: Oleuropein and its peracetylated derivative negatively regulate osteoclastogenesis by controlling the expression of genes involved in osteoclast differentiation and function

Article information

Article type
Paper
Submitted
19 Feb 2020
Accepted
14 Apr 2020
First published
17 Apr 2020
This article is Open Access
Creative Commons BY-NC license

Food Funct., 2020,11, 4038-4048

Oleuropein and its peracetylated derivative negatively regulate osteoclastogenesis by controlling the expression of genes involved in osteoclast differentiation and function

M. A. Rosillo, S. Montserrat-de-la-Paz, R. Abia, M. L. Castejon, M. C. Millan-Linares, C. Alarcon-de-la-Lastra, J. G. Fernandez-Bolaños and F. J. G. Muriana, Food Funct., 2020, 11, 4038 DOI: 10.1039/D0FO00433B

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