Immunochromatographic assay for melamine based on luminescent quantum dot beads as signaling probes

Abstract

To screen and detect the harmful substance melamine (MEL), a quantum-dot-bead-based immunochromatographic assay (QB-ICA) was formulated. After optimization, calibration was performed within the linear range from 0.06 to 0.28 ng mL⁻¹, with limit of detection (LOD) of 0.04 ng mL⁻¹. The LOD was 35 times lower than that of ICA that used colloidal gold nanoparticles (LOD = 1.4 ng mL⁻¹) and 40 times lower than that of the assay based on quantum dots (LOD = 1.6 ng mL⁻¹). In the detection of MEL in spiked pure milk using the proposed QB-ICA strategy, the LOD (LOD = 0.19 ng mL⁻¹) of the samples with the proposed pretreatment was 18.4 times lower than those of the samples without pretreatment (LOD = 3.5 ng mL⁻¹). The performance and practicability of the proposed QB-ICA system was validated; the obtained results reveal that QB-ICA is comparable with the conventional enzyme-linked immunosorbent assay (ELISA) method, but with enhanced applicability. Given its high sensitivity and practicability, the QB-ICA strategy could become a worthwhile alternative for the rapid, sensitive, and quantitative onsite detection of harmful substances, facilitating food safety monitoring.