Interaction among clays and bovine serum albumin

Abstract

Interactions between bovine serum albumin and various clays including pure clay minerals and bentonite were studied with the aim to describe the interaction process. The adsorption of albumin on the clays is strongly affected by the behavior of clays in the aquatic environment (hydrolysis and release of cations). A sufficient amount of albumin was adsorbed on the acid-activated montmorillonite K10 (0.067 mg mg⁻¹) and on the illite–smectite (0.086 mg mg⁻¹). These clay minerals do not strongly affect the sorption solution parameters such as pH value and content of cations. Practically no adsorption was observed on the bentonite and vermiculite. Bentonite and vermiculite are subject to stronger interactions with water which cause the increase of pH value of the sorption solution and release of cations to the solution and thus they cause conformational changes of albumin, which was confirmed by circular dichroism measurements. Obtained results were confirmed by infrared spectroscopy and thermal analysis as well. Interaction of studied materials with bovine serum albumin causes the reduction of particle size in the case of all studied clays except vermiculite. Albumin probably attacks the clay structure during the adsorption, which causes the decrease of particle size. The presented work contributes to the knowledge about interaction of bovine serum albumin with clays in the field of influence of physico-chemical behaviour of clays in the solution on the interaction with albumin.