Design and fabrication of a microfluidic chip to detect tumor markers

Abstract

A microfluidic chip based on capillary infiltration was designed to detect tumor markers. Serum samples flowed along a microchannel that used capillary force to drive sample injection, biochemical reactions and waste liquid collection. This permitted us to realize rapid qualitative detection of tumor markers and other biological molecules. The chip integrated a number of microfluidic functions including blood plasma separation, microvalve operation, and antibody immobilization. Using antigen–antibody reaction principles, the chip provided highly selective and sensitive detection of markers. Combining a microfluidic chip with immunoassays not only improved the antigen–antibody reaction speed, but also reduced the consumption of samples and reagents. The experimental results showed that the chip can achieve separation of trace whole blood, control of sample flow rate, and detection of alpha fetoprotein, thus providing preliminary verification of its feasibility and potential for clinical use. In summary, in this paper a cheap, mass-produced, and portable microfluidic chip for cancer detection, which has good prospects for practical use during disease diagnosis and screening is reported.