Issue 48, 2020

Human serum albumin templated MnO2 nanosheets as an efficient biomimetic oxidase for biomolecule sensing

Abstract

Herein, we have proposed a colorimetric biosensor for detection of acid phosphatase based on human serum albumin (HSA) templated MnO2 nanosheets (HSA-MnO2 NSs). HSA-MnO2 NSs as an efficient biomimetic oxidase could catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) to the coloured oxidation product (oxTMB). Acid phosphatase (ACP) could hydrolyze l-ascorbic acid-2-phosphate (AAP) to produce ascorbic acid, and ascorbic acid could lead to the decomposition of MnO2 NSs to Mn2+ ions, inhibiting the production of oxTMB. On the basis of this, we have demonstrated a novel colorimetric approach for the detection of acid phosphatase with the linear range from 50 μU mL−1 to 1500 μU mL−1 and a detection limit of 40 μU mL−1. The MnO2 NS-based colorimetric method has been successfully used to determine the content of acid phosphatase in real samples with satisfactory results.

Graphical abstract: Human serum albumin templated MnO2 nanosheets as an efficient biomimetic oxidase for biomolecule sensing

Supplementary files

Article information

Article type
Paper
Submitted
19 Jul 2020
Accepted
11 Nov 2020
First published
11 Nov 2020

J. Mater. Chem. B, 2020,8, 11090-11095

Human serum albumin templated MnO2 nanosheets as an efficient biomimetic oxidase for biomolecule sensing

J. Ge, J. Yu, H. Yang, D. Yang and R. Cai, J. Mater. Chem. B, 2020, 8, 11090 DOI: 10.1039/D0TB01766C

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