Issue 4, 2021

Simultaneous measurement of neurite and neural body mass accumulation via quantitative phase imaging

Abstract

Measurement of neuron behavior is crucial for studying neural development and evaluating the impact of potential therapies on neural regeneration. Conventional approaches to imaging neuronal behavior require labeling and do not separately quantify the growth processes that underlie neural regeneration. In this paper we demonstrate the use of quantitative phase imaging (QPI) as a label-free, quantitative measurement of neuron behavior in vitro. By combining QPI with image processing, our method separately measures the mass accumulation rates of soma and neurites. Additionally, the data provided by QPI can be used to separately measure the processes of maturation and formation of neurites. Overall, our approach has the potential to greatly simplify conventional neurite outgrowth measurements, while providing key data on the resources used to produce neurites during neural development.

Graphical abstract: Simultaneous measurement of neurite and neural body mass accumulation via quantitative phase imaging

Supplementary files

Article information

Article type
Paper
Submitted
01 Oct 2020
Accepted
16 Dec 2020
First published
21 Dec 2020
This article is Open Access
Creative Commons BY license

Analyst, 2021,146, 1361-1368

Simultaneous measurement of neurite and neural body mass accumulation via quantitative phase imaging

S. Pradeep, T. Tasnim, H. Zhang and T. A. Zangle, Analyst, 2021, 146, 1361 DOI: 10.1039/D0AN01961E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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