Analysis of proteins and chiral drugs based on vancomycin covalent capillary electrophoretic coating

Abstract

Vancomycin is an amphoteric glycopeptide molecule, and its group diversity and chiral active sites provide a potential basis for its application in chromatographic analysis. In this article, using photosensitive diazo resin (DR) as the coupling agent, vancomycin is modified on the inner wall of the capillary to construct a capillary coating separation system. The highlight of the coated capillary is that it has both anti-protein adsorption and chiral separation properties. Compared with the bare capillary or non-covalently bonded DR/vancomycin-coated capillary, it can not only achieve the separation of four mixed proteins of lysozyme (Lys), bovine serum albumin (BSA), myoglobin (Mb), and ribonuclease A (RNase A), but also shows excellent performance in chiral drugs. The coated capillary effectively solves the problems of low efficiency of the separation column and high sample loss and provides ideas for the development of coated capillaries in the future.