Multimodal chemical imaging of a single brain tissue section using ToF-SIMS, MALDI-ToF and immuno/histochemical staining

Ibrahim Kaya; Eva Jennische; Stefan Lange; Per Malmberg

doi:10.1039/D0AN02172E

Multimodal chemical imaging of a single brain tissue section using ToF-SIMS, MALDI-ToF and immuno/histochemical staining†

Ibrahim Kaya,

*^abcd Eva Jennische,^e Stefan Lange^e and Per Malmberg

*^cf

Author affiliations

* Corresponding authors

^a Department of Psychiatry and Neurochemistry, Sahlgrenska Academy at the University of Gothenburg, Mölndal Hospital, House V, S-43180 Mölndal, Sweden
E-mail: ibrahim.kaya@farmbio.uu.se

^b Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 412 96, Sweden

^c The Gothenburg Imaging Mass Spectrometry (Go:IMS) Platform, University of Gothenburg and Chalmers University of Technology, Gothenburg 412 96, Sweden
E-mail: malmper@chalmers.se

^d Current affiliation: Medical Mass Spectrometry Imaging, Department of Pharmaceutical Biosciences, Uppsala University, BMC 591, Uppsala 751 24, Sweden

^e Institute of Biomedicine, University of Gothenburg, Gothenburg 405 30, Sweden

^f Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg 412 96, Sweden

Abstract

Cluster ion beam ToF-SIMS and/or MALDI-ToF mass spectrometry imaging (using 1,5-DAN matrix via sublimation) of a single coronal rat brain tissue section followed by classical- or immuno- histochemical staining faclilated a new multimodal chemical imaging workflow allowing complementary correlation of the lipid molecular ion images with the immuno/histological features within cerebellum region of the same brain tisue section.

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DOI: https://doi.org/10.1039/D0AN02172E
Article type: Communication
Submitted: 03 Nov 2020
Accepted: 09 Dec 2020
First published: 21 Dec 2020

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Analyst, 2021,146, 1169-1177

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Multimodal chemical imaging of a single brain tissue section using ToF-SIMS, MALDI-ToF and immuno/histochemical staining

I. Kaya, E. Jennische, S. Lange and P. Malmberg, Analyst, 2021, 146, 1169 DOI: 10.1039/D0AN02172E

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Multimodal chemical imaging of a single brain tissue section using ToF-SIMS, MALDI-ToF and immuno/histochemical staining†

Abstract

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Multimodal chemical imaging of a single brain tissue section using ToF-SIMS, MALDI-ToF and immuno/histochemical staining

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