Capture and detection of urine bacteria using a microchannel silicon nanowire microfluidic chip coupled with MALDI-TOF MS

Abstract

Fast bacterial identification in urine samples was achieved by capturing bacteria on a microchannel silicon nanowire microfluidic chip, followed by MALDI-TOF MS detection. Under the optimized conditions, bacteria with a concentration of 10⁶ CFU mL⁻¹ in urine samples could be identified without culture. If cultured for 4 hours, bacteria with a concentration as low as 10³ CFU mL⁻¹ were identified.