Electrochemiluminescence of Ru(bpy)32+/thioacetamide and its application for the sensitive determination of hepatotoxic thioacetamide

Abstract

Thioacetamide (TAA) is a well-known hepatotoxic substance, so it is important to determine its presence and content in food and environmental samples. Herein, we report a highly sensitive determination method for TAA based on the electrochemiluminescence (ECL) of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)₃²⁺) for the first time by using TAA as a new coreactant for Ru(bpy)₃²⁺ ECL via an anodic route. The developed Ru(bpy)₃²⁺–TAA ECL system allows the determination of TAA with a good dynamic linear range and low limit of detection (LOD) of 0.1 μM to 1000 μM and 0.035 μM (3σ/m), respectively. In addition, the established ECL system can be applied to detect TAA in fruit juice and waste water samples with outstanding recoveries.