The exploitation of thermophile resources in hot springs: fluorescent carbon dots derived from Ureibacillus thermosphaericus for multicolour cellular imaging and selectivity detection of heavy metals

Abstract

Microbial biomass, as an environmentally friendly resource, has attracted considerable attention as a green biomaterial for the production of unique and functionalised CDs; however, further exploration is required to characterise CDs derived from bacteria. In this study, a green biomaterial (fluorescence CDs-HS18) was successfully synthesised via a hydrothermal method from Ureibacillus thermosphaericus HS-18 specimens isolated from a hot spring. The prepared CDs-HS18 possess excellent photo-physical properties, outstanding fluorescence capabilities, and high biocompatibility, which make them desirable candidates for multi-mode imaging applications. Our results demonstrate that the prepared CDs can selectively stain the membrane of the biological cells tested and can be rapidly distributed to all parts of the leaf via the veins and intercellular interstitium through transpiration. Additionally, CDs-HS18 are likely to enter the digestive tract of Microworms through ingestion and spread rapidly through the entire body and may finally be excreted through the anus. Furthermore, the rapid and highly selective detection platform based on CDs-HS18 exhibited an excellent linear response for Cr⁶⁺ between 0 and 9 μM, with a detection limit of 36 nM. This research will expand the understanding of the characteristics of green biomaterials derived from bacteria and widen the application scope of hot spring resources.