Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.
J. Machado, R. Ting, J. Y. Lin and E. A. Rodriguez, RSC Chem. Biol., 2021, 2, 1221 DOI: 10.1039/D1CB00127B
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