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In bioimaging, bioorthogonal chemistry is most often used to visualize chemical reporters by fluorescence in their native environment. Herein, we show that TEMPO-based probes can be ligated to monolignol reporters by Diels–Alder chemistry in plant cell walls, paving the way for the study of lignification by EPR spectroscopy and imaging.

Graphical abstract: EPR imaging of sinapyl alcohol and its application to the study of plant cell wall lignification

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