Issue 31, 2021
From the journal:

Chemical Communications

Capturing protein droplets: label-free visualization and detection of protein liquid–liquid phase separation with an aggregation-induced emission fluorogen†

Chu-Qiao Liang,a   Lin Wang,a   Yun-Yi Luo,a   Qian-Qian Lia  and  Yan-Mei Li ORCID logo *abc  

* Corresponding authors

a Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University Beijing, P. R. China
E-mail: liym@mail.tsinghua.edu.cn

b Beijing Institute for Brain Disorders, Beijing 100069, P. R. China

c Center for Synthetic and System Biology, Tsinghua University, Beijing 100084, P. R. China

Abstract

We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of the protein condensed phase based on the lifetime of the DroProbe was also developed.

Graphical abstract: Capturing protein droplets: label-free visualization and detection of protein liquid–liquid phase separation with an aggregation-induced emission fluorogen

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Supplementary files

Article information

DOI
https://doi.org/10.1039/D1CC00947H
Article type
Communication
Submitted
19 Feb 2021
Accepted
08 Mar 2021
First published
10 Mar 2021

Chem. Commun., 2021,57, 3805-3808

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Capturing protein droplets: label-free visualization and detection of protein liquid–liquid phase separation with an aggregation-induced emission fluorogen

C. Liang, L. Wang, Y. Luo, Q. Li and Y. Li, Chem. Commun., 2021, 57, 3805 DOI: 10.1039/D1CC00947H

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