A DNAzyme-mediated target-initiated rolling circle amplification strategy based on a microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at the single-cell level

Chunhuan Xu; Shengyu Chen; Jingjin Zhao; Xiaoshu Luo; Shulin Zhao

doi:10.1039/D1CC04719A

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A DNAzyme-mediated target-initiated rolling circle amplification strategy based on a microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at the single-cell level†

Chunhuan Xu,^a Shengyu Chen,^ab Jingjin Zhao,

*^a Xiaoshu Luo^a and Shulin Zhao

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, School of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin 541004, China
E-mail: jzhao12@163.com, zhaoshulin001@163.com

^b Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, College Chemistry & Environment Engineering, Baise University, Baise 533000, China

Abstract

A DNAzyme-mediated target-initiated rolling circle signal amplification strategy based on a microchip platform was developed for detecting apurinic/apyrimidine endonuclease 1 (APE1) at the single-cell level. This strategy was applied to assays of lysate samples from HL-7702, HeLa and MCF-7 cells, with a detection limit of lower than 1 HeLa cell.

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Article information

DOI: https://doi.org/10.1039/D1CC04719A
Article type: Communication
Submitted: 25 Aug 2021
Accepted: 22 Sep 2021
First published: 22 Sep 2021

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Chem. Commun., 2021,57, 11017-11020

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A DNAzyme-mediated target-initiated rolling circle amplification strategy based on a microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at the single-cell level

C. Xu, S. Chen, J. Zhao, X. Luo and S. Zhao, Chem. Commun., 2021, 57, 11017 DOI: 10.1039/D1CC04719A

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Chemical Communications

A DNAzyme-mediated target-initiated rolling circle amplification strategy based on a microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at the single-cell level†

Abstract

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A DNAzyme-mediated target-initiated rolling circle amplification strategy based on a microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at the single-cell level

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