Issue 17, 2021

Arrow pushing in RNA modification sequencing

Abstract

Methods to accurately determine the location and abundance of RNA modifications are critical to understanding their functional role. In this review, we describe recent efforts in which chemical reactivity and next-generation sequencing have been integrated to detect modified nucleotides in RNA. For eleven exemplary modifications, we detail chemical, enzymatic, and metabolic labeling protocols that can be used to differentiate them from canonical nucleobases. By emphasizing the molecular rationale underlying these detection methods, our survey highlights new opportunities for chemistry to define the role of RNA modifications in disease.

Graphical abstract: Arrow pushing in RNA modification sequencing

Article information

Article type
Review Article
Submitted
27 Feb 2021
First published
14 Jul 2021

Chem. Soc. Rev., 2021,50, 9482-9502

Arrow pushing in RNA modification sequencing

D. Bartee, S. Thalalla Gamage, C. N. Link and J. L. Meier, Chem. Soc. Rev., 2021, 50, 9482 DOI: 10.1039/D1CS00214G

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