Maternal stevioside supplementation ameliorates intestinal mucosal damage and modulates gut microbiota in chicken offspring challenged with lipopolysaccharide

Abstract

Our previous study showed that dietary stevioside supplementation could alleviate intestinal mucosal damage induced by lipopolysaccharide (LPS) through its anti-inflammatory and antioxidant effects in broiler chickens. However, it remains unknown whether feeding stevioside to breeder hens could exert similar biological functions in their offspring. The present study aimed to investigate whether maternal dietary stevioside supplementation could prevent LPS-induced intestinal mucosal damage and alteration of gut microbiota in chicken offspring. A total of 120 Jinmao yellow-feathered breeder hens were fed a basal diet (CON) or a 250 mg kg⁻¹ stevioside-supplemented diet (STE) for 5 weeks before collecting their eggs. After hatching, 160 male offspring (80 chickens from each group) were randomly selected and divided into four treatment groups: (1) the offspring of hens fed a basal diet (CON); (2) the offspring of hens fed a stevioside-supplemented diet (STE); (3) the CON group challenged with LPS (LPS); and (4) the STE group challenged with LPS (LSTE). The results showed that maternal stevioside supplementation increased the hatching weight and improved the intestinal morphology. LPS challenge significantly decreased the terminal body weight and the concentrations of serum triglyceride (TG) and glucose (GLU) of the chicken offspring. Maternal stevioside supplementation protected against LPS-induced morphological damage, goblet cell impairment, intestinal apoptosis, and gene expression alteration. In addition, sequence analysis of 16S rRNA gene showed that maternal stevioside supplementation could prevent the impairment of bacterial diversity in LPS-challenged chicken offspring. Moreover, the increased abundance of Lactobacillus caused by maternal stevioside supplementation had a significant negative correlation with the expression of intestinal inflammatory cytokines. In conclusion, maternal stevioside supplementation could ameliorate intestinal mucosal damage and modulate gut microbiota in chicken offspring challenged with LPS.