Issue 14, 2021

Auto-Panning: a highly integrated and automated biopanning platform for peptide screening

Abstract

Biopanning, a common affinity selection approach in phage display, has evolved numerous ligands for diagnosis, imaging, delivery, and therapy applications. However, traditional biopanning has suffered from time-consuming processes, highly-repetitive procedures and labor-intensive manual operation. Herein, a highly integrated and automated biopanning platform (Auto-Panning) is proposed. Based on digital microfluidics (DMF), biopanning processes are integrated on a chip with highly reproducible, precise, automated liquid manipulation. Therefore, 3 rounds of Auto-Panning can be accomplished within 16 h, instead of nearly a week of complicated manual operations. Auto-Panning has been used to evolve a specific peptide against cancer biomarker EphA2 with excellent cellular penetrating ability and significant invasion suppression biofunction, successfully demonstrating the practicality of the platform. Overall, as an automated programmable molecular screening platform, Auto-Panning will further promote the discovery and applications of novel ligands.

Graphical abstract: Auto-Panning: a highly integrated and automated biopanning platform for peptide screening

Supplementary files

Article information

Article type
Paper
Submitted
19 Feb 2021
Accepted
01 Jun 2021
First published
02 Jun 2021

Lab Chip, 2021,21, 2702-2710

Auto-Panning: a highly integrated and automated biopanning platform for peptide screening

J. Wang, J. Guo, K. Zhao, W. Ruan, L. Li, J. Ling, R. Peng, H. Zhang, C. Yang and Z. Zhu, Lab Chip, 2021, 21, 2702 DOI: 10.1039/D1LC00129A

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