Issue 2, 2021
From the journal:

Organic & Biomolecular Chemistry

Mechanistic divergence between (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol synthases from Dictyostelium purpureum and Streptomyces coelicolor†

Houchao Xu,a   Jan Rinkel,a   Xinlu Chen,b   Tobias G. Köllner,c   Feng Chen ORCID logo b  and  Jeroen S. Dickschat ORCID logo *a  

* Corresponding authors

a Kekulé-Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Strasse 1, 53121 Bonn, Germany
E-mail: dickschat@uni-bonn.de

b Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN 37996-4561, USA

c Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745 Jena, Germany

Abstract

The main product of DpTPS9 from the social amoeba Dictyostelium purpureum was identified as (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol that is also known as an intermediate of bacterial geosmin synthase, but the experimentally verified cyclisation mechanisms differ. Together with the low sequence identity this points to convergent evolution. The functionality of selected residues in DpTPS9 was investigated via site-directed mutagenesis experiments.

Graphical abstract: Mechanistic divergence between (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol synthases from Dictyostelium purpureum and Streptomyces coelicolor

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Article information

DOI
https://doi.org/10.1039/D0OB02361B
Article type
Communication
Submitted
26 Nov 2020
Accepted
15 Dec 2020
First published
15 Dec 2020

Org. Biomol. Chem., 2021,19, 370-374

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Mechanistic divergence between (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol synthases from Dictyostelium purpureum and Streptomyces coelicolor

H. Xu, J. Rinkel, X. Chen, T. G. Köllner, F. Chen and J. S. Dickschat, Org. Biomol. Chem., 2021, 19, 370 DOI: 10.1039/D0OB02361B

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