Issue 1, 2021, Issue in Progress

Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast

Abstract

The need of identifying alternative therapeutic targets for invasive ductal carcinoma (IDC) of the breast with high specificity and sensitivity for effective therapeutic intervention is crucial for lowering the risk of fatality. Lipidomics has emerged as a key area for the discovery of potential candidates owing to its several shared pathways between cancer cell proliferation and survival. In the current study, we performed comparative phospholipidomic analysis of IDC, benign and control tissue samples of the breast to identify the significant lipid alterations associated with malignant transformation. A total of 33 each age-matched tissue samples from malignant, benign and control were analyzed to identify the altered phospholipids by using liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM/MS). A combination of univariate and multivariate statistical approaches was used to select the phospholipid species with the highest contribution in group segregation. Furthermore, these altered phospholipids were structurally confirmed by tandem mass spectrometry. A total of 244 phospholipids were detected consistently at quantifiable levels, out of which 32 were significantly altered in IDC of the breast. Moreover, in pairwise comparison of IDC against benign and control samples, 11 phospholipids were found to be significantly differentially expressed. Particularly, LPI 20:3, PE (22:1/22:2), LPE 20:0 and PC (20:4/22:4) were observed to be most significantly associated with IDC tissue samples. Apart from that, we also identified that long-chain unsaturated fatty acids were enriched in the IDC tissue samples as compared to benign and control samples, indicating its possible association with the invasive phenotype.

Graphical abstract: Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast

Article information

Article type
Paper
Submitted
27 Aug 2020
Accepted
27 Nov 2020
First published
22 Dec 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2021,11, 397-407

Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast

R. Taware, T. H. More, M. Bagadi, K. Taunk, A. Mane and S. Rapole, RSC Adv., 2021, 11, 397 DOI: 10.1039/D0RA07368G

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