Chemical characterization of red cells from the black sea urchin Arbacia lixula by X-ray photoelectron spectroscopy

Abstract

Red spherula cells (RSC) from sea urchin coelomic fluid have attracted great interest for their specific and intriguing properties, such as for example antimicrobial activities and immune response, that probably tie in with their red characteristic pigments. Although to date different studies have been reported aimed to chemically characterize their pigments extracted from the cells, few data are available about the chemical characterization of the cell surface. In this work, a systematic chemical characterization of the RSC surface by X-ray photoelectron spectroscopy (XPS) analysis is described. The results were compared with data on colorless cells from the same coelomic fluid sample. Our observations evidenced that the two cell types were characterized by the presence of different chemical functional groups. In particular, the colorless cells are dominated by the presence of alkyl, alcohol, amide, and carboxyl groups in accordance with other similar cell types, enriched in Na⁺ and Cl⁻ ions. Traces of elements like S (sulphonates) and P (phosphates) are also present. On the other hand, the RSC in addition to the alkyl groups show a reduction in the content of amide groups, accompanied by the anomalous presence of keto-enolic groups that probably can be associated with the presence of quinones/hydro-quinones from red pigments. A chemical enrichment in elements such as Cl⁻ and Mg²⁺ and sulphate groups (–R–O–SO₃⁻), as well as the presence of sulphides and phosphates traces, is evident. The absence of carbonate groups is also observed in both cell populations, confirming the absence of sodium and magnesium carbonate salts. No traces of toxic elements (i.e., heavy metals) have been revealed.