Rapid mapping of the IAA in leaves of Arabidopsis thaliana using a simple paper-based electroanalytical device coupled with microsampling

Abstract

To deeply investigate the pivotal roles of Auxin (mainly indole-3-acetic acid, IAA), it is essential to obtain the contents of IAA in different locations of plants. It is still a challenge to quantify the levels of IAA in different sites of Arabidopsis thaliana leaves because of the small sizes. In this study, a simple paper-based electroanalytical device coupled with microsampling was used to differentiate the IAA amounts in different locations of Arabidopsis thaliana leaves. For the micro real sampling, the different areas of the thaliana leaves were retrieved by the Harris Uni-Core TM Miltex® with diameters: 1.0, 1.5, 2.5, 3.5, and 4.0 mm. The results showed that the contents of IAA can be detected from circle samples with the diameter from 1.0 to 4.0 mm. With 1.5 mm diameter sampling, the levels of IAA could be obtained in different sites of cotyledon and the first true leaf of Arabidopsis thaliana at the seedling stage. Our results suggested that the highest IAA levels were in the near petiole and lowest IAA levels in the leaf tip, which roughly agreed with those in tobacco leaves based on HPLC-MS reported before. In addition, the microsampling has a minor impact on the growth of Arabidopsis thaliana in the following especially for circle samples with the diameter 1.5 mm. This study revealed the potential application of microsampling coupled with a simple paper-based electroanalytical device for the mapping study of IAA in small plants or small tissue samples.