Biomarker imprinted magnetic core–shell nanoparticles for rapid, culture free detection of pathogenic bacteria

Abstract

Rapid and selective detection of microorganisms in complex biological systems draws huge attention to address the rising issue of antimicrobial resistance. Diagnostics based on the identification of whole microorganisms are laborious, time-consuming and costly, thus alternative strategies for early clinical diagnosis include biomarker based microbial detection. This paper describes a low-cost, easy-to-use method for the detection of Pseudomonas aeruginosa infections by specifically identifying a biomarker pyocyanin, using surface-molecularly imprinted nanoparticles or “plastibodies”. The selective nanopockets are created by templating pyocyanin onto 20 nm allyl-functionalized magnetic nanoparticles coated with a thin layer of the acrylamide-based polymer. This functional material with an impressive imprinting factor (IF) of 5 and a binding capacity of ∼2.5 mg g⁻¹ of polymers can be directly applied for the detection of bacteria in complex biological samples based on the presence of pyocyanin. These MIPs are highly selective and sensitive to pyocyanin and can consistently bind with pyocyanin in repeated use. Finally, the facile and efficient capture of pyocyanin has versatile applications ranging from biomarker based culture free detection of P. aeruginosa to monitoring of the therapeutic regime, in addition to developing a new class of antibiotics.