Issue 18, 2022

Fusogenic peptide modification to enhance gene delivery by peptide-DNA nano-coassemblies

Abstract

Endosomal escape is a major obstacle for non-viral nucleic acids delivery. Here, we attached by click reaction a fusogenic peptide (L17E) onto peptide self-assembled disks (∼17 nm), which mimicked the functional subunits of the virus capsid. These peptide disks then spontaneously co-assembled with DNA to form patterned nanostructures (∼100 nm) as viral mimics. This modification did not affect the cellular uptake but enhanced endosomal escape and led to improved transfection in cell culture.

Graphical abstract: Fusogenic peptide modification to enhance gene delivery by peptide-DNA nano-coassemblies

Associated articles

Supplementary files

Article information

Article type
Communication
Submitted
07 May 2022
Accepted
30 Jul 2022
First published
01 Aug 2022

Biomater. Sci., 2022,10, 5116-5120

Fusogenic peptide modification to enhance gene delivery by peptide-DNA nano-coassemblies

R. Feng, R. Ni and Y. Chau, Biomater. Sci., 2022, 10, 5116 DOI: 10.1039/D2BM00705C

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