Silver loaded biodegradable carboxymethyl chitin films with long-lasting antibacterial activity for infected wound healing

Abstract

Bacteria-related infections are one of the main causes of human skin infections, which are associated with the delay of wound healing and secondary complications. In this work, a series of novel biodegradable films based on thermosensitive carboxymethyl chitin were prepared without using any crosslinkers. All the carboxymethyl chitin films had good flexibility, high transparency, and appropriate water absorption capacity, and could provide a moist environment for wound healing. The silver ions (Ag⁺) were incorporated on the LTCF-5 film, which had the best mechanical strength (56.39 MPa in the dry state and 0.66 MPa in the wet state) among the carboxymethyl chitin films and was higher than those of the reported biodegradable dressings and commercially available dressings. Compared with the commercial hydrofiber dressing with silver (AQUACEL®), the composite film could provide slow and sustained release of Ag⁺ with good strength and biodegradability, and displayed excellent long-lasting antibacterial activity in vitro against both S. aureus and E. coli without obvious cytotoxicity, which still possessed good antibacterial activity with almost 100% bacteriostatic rates after soaking in phosphate buffered saline for 7 days. More importantly, the Ag⁺ loaded carboxymethyl chitin film could promote infected cutaneous wound healing in a S. aureus infected full-thickness cutaneous defect in vivo model because of its long-lasting antibacterial activity, good biocompatibility, exudate absorption and ability to maintain a moist environment. Thus Ag⁺ loaded carboxymethyl chitin films are excellent candidates for infected wound healing.