Issue 34, 2022
From the journal:

Chemical Communications

In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions†

Takumi Yokoyama,‡a   Takehiro Ando,‡a   Yukio Takamori,‡a   Daisuke Fuji,‡a   Masashi Sato,a   Santhana Vedi,a   Mizuki Yamamotoa  and  Takashi Kawakami ORCID logo *a  

* Corresponding authors

a Department of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Yamanashi 400-8510, Japan
E-mail: tkawakami@yamanashi.ac.jp

Abstract

We report novel, ribosomally incorporatable, and intramolecularly cysteine-reactive fluorobenzoic acid-derived linkers for SELEX of mRNA-displayed unnatural peptides, which spontaneously cyclized via intramolecular nucleophilic aromatic substitutions forming thioethers. With this strategy we identified several novel PCSK9-binding peptides.

Graphical abstract: In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions

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Article information

DOI
https://doi.org/10.1039/D2CC00584K
Article type
Communication
Submitted
01 Feb 2022
Accepted
25 Mar 2022
First published
07 Apr 2022

Chem. Commun., 2022,58, 5237-5240

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In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions

T. Yokoyama, T. Ando, Y. Takamori, D. Fuji, M. Sato, S. Vedi, M. Yamamoto and T. Kawakami, Chem. Commun., 2022, 58, 5237 DOI: 10.1039/D2CC00584K

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