Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

Soei Watari; Hiroshi Inaba; Tomonori Tamura; Arif Md. Rashedul Kabir; Akira Kakugo; Kazuki Sada; Itaru Hamachi; Kazunori Matsuura

doi:10.1039/D2CC01890J

Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide†

Soei Watari,^a Hiroshi Inaba,

*^ab Tomonori Tamura,

^c Arif Md. Rashedul Kabir,

^d Akira Kakugo,

^de Kazuki Sada,^de Itaru Hamachi

^cf and Kazunori Matsuura

*^ab

Author affiliations

* Corresponding authors

^a Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan
E-mail: hinaba@tottori-u.ac.jp, ma2ra-k@tottori-u.ac.jp

^b Centre for Research on Green Sustainable Chemistry, Tottori University, Tottori 680-8552, Japan

^c Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

^d Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan

^e Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan

^f JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience, Nishikyo-ku, Kyoto 615-8530, Japan

Abstract

For light-induced stabilization of microtubules (MTs) to manipulate cells, a photo-reactive diazirine group was conjugated to a Tau-derived peptide, a motif binding on the inside of MTs. Ultraviolet (UV) light irradiation induced significant stabilization of MTs via the formation of a covalent bond of the peptide and showed toxicity.

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DOI: https://doi.org/10.1039/D2CC01890J
Article type: Communication
Submitted: 02 Apr 2022
Accepted: 25 Jul 2022
First published: 05 Aug 2022
This article is Open Access

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Chem. Commun., 2022,58, 9190-9193

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Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

S. Watari, H. Inaba, T. Tamura, A. Md. R. Kabir, A. Kakugo, K. Sada, I. Hamachi and K. Matsuura, Chem. Commun., 2022, 58, 9190 DOI: 10.1039/D2CC01890J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide†

Abstract

Supplementary files

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Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

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